| M-400m | Gunnar Lewis | Raceland | 50.97 | 1 |
| M-400m | Bryce Donnelly | Bishop Brossart | 51.32 | 1 |
| M-400m | Ethan Brown | West Carter | 51.34 | 2 |
| M-400m | Mitchell Otten | Newport Central Catholic | 51.77 | 2 |
| M-400m | Jaylyn McMurry | Russellville | 51.8 | 1 |
| M-400m | Will Tolley | Crittenden County | 51.99 | 1 |
| M-400m | Hayden Norris | St. Henry District | 52.07 | 3 |
| M-400m | Cabot Cecil | Walden | 52.45 | 1 |
| M-400m | Kj Crump | University Heights | 52.5 | 2 |
| M-400m | Chris Knuckles | The Academy @ Shawnee | 52.71 | 2 |
| M-400m | Josh Clark | Raceland | 52.87 | 3 |
| M-400m | Julio Zepeda | Nicholas County | 52.98 | 1 |
| M-400m | Nick Parker | Bracken County | 53.17 | 2 |
| M-400m | Christian Acevedo | Fort Knox | 53.67 | 3 |
| M-400m | Spencer Barrett | Walden | 53.68 | 4 |
| M-400m | Trevor Kahmann | Bishop Brossart | 53.7 | 4 |
| M-400m | Logan Potter | Lexington Christian | 53.77 | 3 |
| M-400m | Kamoni Green | Fort Knox | 53.78 | 5 |
| M-400m | Patrick Schepers | Owensboro Catholic | 53.9 | 2 |
| M-400m | Bryant Feck | Model | 53.99 | 1 |
| M-400m | Bricklen Avdevich | Holy Cross (Louisville) | 54.11 | 6 |
| M-400m | Ryan Samotis | St. Henry District | 54.25 | 5 |
| M-400m | Cameron Buck | Hancock County | 54.44 | 3 |
| M-400m | Jacob Bailey | Owensboro Catholic | 54.44 | 4 |
| M-400m | Steven Poore | Middlesboro | 54.76 | 2 |