| Girls Seeded Race | ||
| # | Team (ST) | Division |
| 1 | Assumption high School (KY) | Southeast |
| 2 | Brentwood Academy (TN) | Southeast |
| 3 | Brentwood High School (TN) | Southeast |
| 4 | Carmel High School (IN) | Midwest |
| 5 | Central Magnet High School (TN) | Southeast |
| 6 | Christian Academy of Knoxville (TN) | Southeast |
| 7 | Daviess County High School (KY) | Southeast |
| 8 | Dublin Jerome High School (OH) | Midwest |
| 9 | duPont Manual High School (KY) | Southeast |
| 10 | Floyd Central High School (IN) | Midwest |
| 11 | Highlands High School (KY) | Southeast |
| 12 | Liberty-Benton High School (OH) | Midwest |
| 13 | Male High School (KY) | Southeast |
| 14 | Mason High School (OH) | Midwest |
| 15 | Medina High School (OH) | Midwest |
| 16 | Morristown-West High School (TN) | Southeast |
| 17 | North Oldham High School (KY) | Southeast |
| 18 | Olentangy Orange High School (OH) | Midwest |
| 19 | Ravenwood High School (TN) | Southeast |
| 20 | Riverdale High School (TN) | Southeast |
| 21 | Sacred Heart Academy (KY) | Southeast |
| 22 | Seton High School (OH) | Midwest |
| 23 | Ursuline Academy (OH) | Midwest |
| 24 | West Lafayette High School (IN) | Midwest |